Technologies

TECHNOLOGIES

??TECHNOLOGIES

  • Full-length cDNA technology

Full-length cDNA technology

This is a series of technologies developed by RIKEN for preparing full-length cDNA. Extension, selection, normalization, and new cloning vectors have been developed and are being applied in many fields.

CAGE (Cap Analysis of Gene Expression)

cage

This is a technology for genome-wide survey of transcription start site combining heat-resistant reverse transcriptase and the cap-trapper method to clip out the initial 20 nucleotides from the 5' end mRNAs. By using new generation high speed sequencing technology, CAGE can be used for quantitative gene expression analysis.


Protein-protein interaction assay

Protein-protein mutual interaction (M2H)

High-throughput detection of protein-protein interactions in mammalian cells

Smart Amp (SMAP, SMart Amplification Process)

SMAP

This is an isothermal DNA amplification method developed at RIKEN. Using multiple enzymes and unique primer sets, a single nucleotide polymorphism (SNP) can be precisely detected while amplifying DNA. It can amplify only desired DNA from clinical samples such as blood without purification within 15-30 minutes.


The DNA Book

The DNA Book

This is a simplified technique for storing and delivering genes with their information. In the DNABook, DNAs are spotted on water-soluble paper using a DNA printer. The spotted and dried DNA is stable at room temperature. DNA is easily eluted from any spot and recovered by PCR or transformation of E. coli cells. Several tens of thousands of clones can be stored on a bookshelf. There is no need for a freezer .

Microarray

Microarray

This is a microarray on a glass slide of full-length mouse cDNA developed by RIKEN.


RLGS (Restriction Landmark Genomic Scanning)

RLGS

This is a useful method for genome analysis that uses restriction enzyme sites as landmarks. Originally developed by RIKEN, it is one of the most successfully applied methods for the identification of aberrant CpG island hypermethylation in cancer, as well as the identification of tissue specific methylation of CpG islands.